广州健仑生物科技有限公司

快速检测试试纸(胶体金法)
  • 发布日期: 2019-05-16
  • 更新日期: 2022-05-05
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保质期 个月

咖啡碱快速检测试试纸(胶体金法)


【包装规格】 1人份/袋、40人份/盒

【储存条件及有效期】储存条件:原包装应储存于4~30℃避光干燥处,切忌冷冻。有效期:24个月。

[intended use]

Caffeine, also known as caffeine, is a methyl xanthine compound. Its chemical name is 1, 3, 7-trimethyxanthine, which is caffeine but is highly addictive.To this end, countries in the development of caffeine in beverage food hygiene standards.The United States, Canada, Argentina, Japan, the Philippines in drinks caffeine content must not exceed 200 mg/L, for the former Yugoslavia provisions shall not exceed 120 mg/L, so far our country join the cola beverages allowed only caffeine, its content must not exceed 150 mg/Kg, in order to strengthen food hygiene supervision and management, the determination methods of rapid detection of caffeine is necessary.

【 principle of test 】

This product USES the competitive inhibition method and the principle of colloidal gold immunochromatography to qualitatively detect the caffeine in urine. The monoclonal antibody of gold standard caffeine is used as the indicator marker, and the detection line and the control line of the cellulose nitrate film are respectively coated with caffeine-bsa conjugate and sheep anti-mouse IgG polyclonal antibody.At the time of detection, the urine sample was analyzed under the capillary effect.For example, when the concentration of caffeine in the urine sample is lower than 300ng/mL, the gold-standard antibody cannot all bind with caffeine. The unbound gold-standard antibody binds with the caffeine-bsa binding fixed on the detection line during the chromatography process, and thus a purplish red band appears in the detection area (T).If the concentration of caffeine in the urine sample is higher than 300ng/mL, all the jinbiao antibodies are bound to caffeine, so that in the test area (T), because the competitive reaction will not bind to the caffeine-bsa binding compound, there will be no purplish red band.A purplish red band appears in the control area (C) regardless of the presence or absence of caffeine in the urine sample.The purplish red bands shown in the control area (C) are the standard for determining whether there is sufficient urine sample and whether the chromatography process is normal, and also serve as the internal control standard for reagents.



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