Common staining methods include simple staining, negative staining, gram staining, spore staining, flagellar staining, decidua staining, and life-saving staining. Preparation of bacterial stained tablets generally go through smears, fixation, staining, washing, drying, and other steps, and then use a microscope and even oil mirror observation.

Simple staining:

       Different bacteria or because of the observer's focus on different observations, so there are differences in the use of dyes, but the method of simple staining is the same. First, according to the above-mentioned production method to make tablets, after making slides that need to be observed, use the corresponding dyes to be added to the pellicle membrane area on the slides to cover the pellicles. According to the requirements of different dyes, the dyeing time is determined in conjunction with the observed contents, and the steps of water washing and drying are performed when the dyeing time arrives. The resulting glass slides are covered with coverslips for microscopic examination. If necessary, subsequent sealing process such as oil seal and wax seal can be performed.

Negative staining:

      It is very easy to prepare observation pictures, but it is still difficult for beginners to find the target bacteria in the slides, because bacteria are often colorless, transparent and relatively small, so for beginners, unless the aperture, etc. Very fine adjustment, otherwise it is difficult to observe the target bacteria, so negative staining is very important. In this method, microorganisms are mixed with nigrosine or india ink, and then the mixture is covered on the surface of the glass slide. Since these two natural black dyes cannot infiltrate into microbial cells, transparent individual microorganisms are easily observed under a black background. There are two common methods of negative staining. The first method is more common. Microorganisms are mixed with alanine black on a glass slide and the mixture is evenly covered on the original glass slide with another glass slide. The purpose of this method is to make the mixture thick on one side of the glass slide, and the area of interest in the middle of the thick and thin is the best viewing area.