Shigella polyvalent 3 (12-15)鲍氏志贺菌多价3（12－15）
产品规格
保存要求：除了有特殊说明，免疫检测产品应保存在2-8°C
产品规格：2ml/瓶
保质期：2年
本试剂盒主要用于对病菌细菌进行检测，利用玻片和试管凝集方法鉴定志贺氏菌单价血清和多价血清。
分离培养
取粪便（粘液或脓血部分）或肛拭标本接种GN肉汤增菌，增菌后进行分离培养。一般同时接种强弱选择性不同的两个平板。强选择鉴别培养基可用沙门、志贺菌选择培养基（SS）；若选择诊断血清可选择天津生物芯片福氏志贺式多价诊断血清可疑菌落进行鉴定。
鉴定
挑选可疑菌落3~4个先用志贺菌属多价诊断血清作试探性玻片凝集试验。将试探性凝集试验阳性的菌落至少接种2~3支KIA和MIU，经35 ℃培养18~24h，凡符合；KIA：K/A、产气-/+、H2S-；MIU：动力-、吲哚+/-、尿酶-，并结合试探性玻片凝集试验阳性结果可鉴定为志贺菌属。
核酸扩增法
核酸扩增技术，即聚合酶链式反应（polymerase chainreaction，PCR），其基本原理是设计、合成两条寡核苷酸，作为引物，对应于待测病原微生物某一段特异性序列的两端，然后在体外模拟DNA体内复制的过程反复扩增，使靶序列放大上万倍甚至上百万倍而被检测出来。
中国检验医学暨输血仪器试剂博览会之广州健仑生物
本届展会四方学者齐聚，业内领军精英云集，让我们拓展了视野，丰富了阅历，饱满了人生。我们感激行业里的老师与前辈，感激他们传授我们知识，让我们拥有学习的能力；我们感激一直支持和鼓励我们的客户，感激他们一直默默的帮助，让我们在“星星之火”的路上不断进取与创新；我们感激一直研发与在路上成长同伴们，因为你们让我们团队更加丰富与勇敢地担当我们的社会责任。感谢一直关注健仑和陪伴健仑成长的朋友们。闻道有先后，术业有专攻，广州健仑生物科技公司将吸取CACLP春季会上丰富的知识，进而更好的服务于体外诊断行业的各个领域，为推动体外诊断产业不断进步而努力。致谢！
我司还有很多种血清学诊断血清、血液检测、免疫检测产品、毒素检测、凝集检测、酶免检测、层析检测、免疫荧光检测产品，欢迎致电。
（MOB：13802525278杨永汉）
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健仑图集
Shigella has K and O antigens but no H antigen. The K antigen is a bacterial surface antigen of some strains newly isolated from patients and is not heat-resistant. It is destroyed by heating at 100°C for 1 hour. The K antigen has no significance in serological typing bu
Isolation and cultivation
The GN broth was inoculated with feces (mucus or pus) or anal swabs to enrich bacteria, and was isolated and cultured after enrichment. Two plates with different strength and selectivity are generally inoculated simultaneously. Strong selection of differentiation media available Salmonella, Shigella selection medium (SS); if the choice of diagnostic serum can choose Tianjin Biochip Fugitive Shiga multivalent diagnostic serum suspicious colonies for identification.
Identification
A total of 3 to 4 suspicious colonies were selected for the trial slide agglutination test using Shigella polyvalent diagnostic serum. Colonies that were positive for exploratory agglutination test were inoculated with at least 2 to 3 KIA and MIU, and cultured at 35 °C for 18 to 24 hours. Wherever they meet; KIA: K/A, gas production -/+, H2S-; MIU: dynamic -,哚+/-, urine enzyme-, combined with positive results of exploratory slide agglutination test can be identified as Shigella.
Nucleic Acid Amplification
The principle of nucleic acid amplification, polymerase chain reaction (PCR), is to design and synthesize two oligonucleotides as primers that correspond to the two ends of a specific sequence of the pathogenic microorganism to be tested. Then it replicates in vitro to mimic the in vivo replication of DNA, allowing the target sequence to be amplified tens of times or even millions of times.
