尼古丁快速筛查试剂盒（创仑）
【包装规格】 1人份/袋、40人份/盒
本品采用竞争抑制法和胶体金免疫层析技术，用于定性检测人体尿液中尼古丁，适用于尼古丁药物滥用的初步筛查。
【储存条件及有效期】
储存条件：原包装应储存于4～30℃避光干燥处，切忌冷冻。
有效期：24个月。
试剂盒应在铝箔袋拆封后1小时内尽快使用；建议在周围温度高于30℃或高湿度条件下，尽可能做到即开即用。
intended use
Nicotine is an addictive alkaline chemical found in the leaves of the tobacco plant nicotiana.It is ingested through cigarette inhalation or chewing tobacco.Nicotine is metabolized by the liver into more than 20 compounds that are excreted by the kidneys into the urine.Both smokers and secondhand smokers have elevated levels of nicotine and its primary metabolite, cotinine.Levels of nicotine replacement drugs, such as nicotine tablets or lozenge, also rose.Large doses of nicotine are toxic.
Cotinine, a major metabolite of nicotine, is often selected for evaluation of tobacco use or secondhand smoke exposure because it is stable and occurs only during nicotine metabolism.Cotinine has a half-life of 7 to 40 hours in the body, while nicotine has a half-life of 1 to 4 hours.Blood and/or urine cotinine testing should be done in conjunction with nicotine testing.In some cases, other metabolites of nicotine, such as nicotine-1-nitric oxide, trans-3-hydroxycotinine, nornicotinoid, or other tobacco chemicals, such as quinoa in urine samples, can also be detected.The nicotine and cotinine tests are also used when nicotine poisoning is suspected.Acute nicotine overdose, such as accidental ingestion of nicotine lozenges or chewing gum in children, is relatively rare but requires urgent medical attention.Symptoms include burning sensation in the mouth, nausea, abdominal pain, salivation, diarrhea, sweating, confusion, dizziness, excitement, increased heart rate, shortness of breath or difficulty, epilepsy, coma and even death.
inspection principle
This product USES the competitive inhibition method and the principle of colloidal gold immunochromatography to qualitatively detect the nicotine in the urine. The monoclonal antibody of gold standard nicotine is used as the indicator marker, and the detection line and the control line on the nitrocellulose film are respectively coated with nicotine-bsa conjugate and sheep anti-mouse IgG polyclonal antibody.At the time of detection, the urine sample was analyzed under the capillary effect.For example, when the concentration of nicotine in the urine sample is lower than 300ng/mL, the gold-standard antibody cannot fully bind to nicotine. The unbound gold-standard antibody binds to the nicotine-bsa binding fixed on the detection line during the chromatography process, and thus a purplish red band appears in the detection area (T).If the concentration of nicotine in the urine sample is higher than 300ng/mL, all the gold standard antibodies will bind to nicotine, so that the purple band will not appear in the detection area (T) because the competitive reaction will not bind to the nicotine-bsa binding compound.A purplish red band appears in the control area (C) regardless of the presence or absence of nicotine in the urine sample.The purplish red bands shown in the control area (C) are the standard for determining whether there is sufficient urine sample and whether the chromatography process is normal, and also serve as the internal control standard for reagents.
inspection principle
This product USES the competitive inhibition method and the principle of colloidal gold immunochromatography to qualitatively detect the nicotine in the urine. The monoclonal antibody of gold standard nicotine is used as the indicator marker, and the detection line and the control line on the nitrocellulose film are respectively coated with nicotine-bsa conjugate and sheep anti-mouse IgG polyclonal antibody.At the time of detection, the urine sample was analyzed under the capillary effect.For example, when the concentration of nicotine in the urine sample is lower than 300ng/mL, the gold-standard antibody cannot fully bind to nicotine. The unbound gold-standard antibody binds to the nicotine-bsa binding fixed on the detection line during the chromatography process, and thus a purplish red band appears in the detection area (T).[inspection principle]
This product USES the competitive inhibition method and the principle of colloidal gold immunochromatography to qualitatively detect the nicotine in the urine. The monoclonal antibody of the gold standardIs used as the indicator,And the detection line and the control line on the nitrocellulose qualities are respectively coated with nicotine - bsa conjugate and sheep anti - mouse IgG polyclonal antibody. At the time of detection,The urine sample was analyzed under the capillary effect.For example, when the concentration of flavour in the urine sample is lower than 300ng/mL.The gold - standard antibody always fully bind to nicotine. The unbound gold - standard antibody binds to the nicotine - bsa binding fixed on the detection line during the chromatography process.And thus a purplish red band appears in the detection area (T). If the concentration of nicotine in the urine sample is who 300 ng/mL, all the gold standard antibodies will bind to nicotine,So that the purple band will not appear in the detection area (T) because the competitive reaction will not bind to the bsa binding compound.A purplish red band appears in the control area(C) regardless of the presence or absence of urine sample.The purplish red bands are shown in the control area (C) the standard for determining whether there is sufficient urine sampleAnd been the chromatography process is normal, and also serve as the internal control standard for reagents, such as nicotine concentration in urine is higher than 300 ng/mL, jinbiao antibodies with nicotine, thus in the test region (T) because competition reaction will not combine with nicotine - BSA binders and not a purple stripe.A purplish red band appears in the control area (C) regardless of the presence or absence of nicotine in the urine sample.The purplish red bands shown in the control area (C) are the standard for determining whether there is sufficient urine sample and whether the chromatography process is normal, and also serve as the internal control standard for reagents.
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Tel:020-82574011 Mr.Yang
